Establishment of indirect ELISA diagnose based on the VP1 structural protein of foot-and-mouth disease virus (FMDV) in pigs / 生物工程学报
Chinese Journal of Biotechnology
; (12): 961-966, 2007.
Article
in Zh
| WPRIM
| ID: wpr-276179
Responsible library:
WPRO
ABSTRACT
The complete gene encoding the structural protein of FMDV(VP1) was subcloned into expression vector pPROex-HT, resulting in the fusion expression plasmid pPROexHT-VP1. After transformed into E. coli BL21(DE3) and induced by IPTG, the fusion protein was expressed in high level. Western blot was performed to confirm that the expressed fusion protein could specifically react with antiserum against FMDV. Based on the fusion protein further purified, a novel indirect ELISA (VP1-ELISA) was developed to detect FMDV antibody in pigs. Comparison between VPl-ELISA and the government standard kit (liquid phase block ELISA) showed the two methods had 96.25 percent agreement by detecting 80 serum samples, indicating that the indirect VP1-ELISA was specific and sensitive.
Full text:
1
Database:
WPRIM
Main subject:
Swine
/
Blood
/
Recombinant Fusion Proteins
/
Enzyme-Linked Immunosorbent Assay
/
Sensitivity and Specificity
/
Foot-and-Mouth Disease Virus
/
Capsid Proteins
/
Diagnosis
/
Allergy and Immunology
/
Escherichia coli
Type of study:
Diagnostic_studies
Limits:
Animals
Language:
Zh
Journal:
Chinese Journal of Biotechnology
Year:
2007
Document type:
Article